miR-125b, one of neuronal miRNAs, recently was found to be necessary for stem cell fission to bypass the normal G1/S checkpoint and make stem cells insensitive to chemotherapy signals, which normally stop the cell cycle at the G1/S transition. Given the insensitivity of gliomas to chemotherapy and the hypothesis that glioma stem cells cause resistance to drug therapy, exploring the functions and mechanisms of miR-125b in glioma stem cells would be valuable. This miRNA sequence is predicted based on homology to a verified miRNA from mouse. Its expression was later verified in human BC-1 cells.